(CNN) — They've given up their ground before -- voluntarily, as a political concession. But that seems to be over.
After the deaths of 25 people from clashes a day earlier, Ukrainian protesters are prepared to stand and fight again Wednesday.
Police want to clear them out of central Kiev. Some of them died trying to stay put Tuesday -- using projectiles and burning barricades to keep security forces at bay at Kiev's Maidan, or Independence Square.
It was the deadliest day in the months-long standoff between the government and opposition leaders. Police and protesters died. A journalist and a government employee did, too.
More than 240 others were hospitalized, Ukraine's health ministry said.
Overnight, demonstrators stocked up, passing stones hand to hand, filling Molotov cocktails and stoking flaming barricades with wood and tires.
They prepared a makeshift compressed-air cannon to catapult the projectiles into police ranks.
Flames and smoke were still climbing into the sky after the sun came up.
Ukraine's President Viktor Yanukovych fired fresh vitriol at his opposition Wednesday, pinning blame for the violence exclusively on protesters.
He would have none of it himself.
"This is my life principle - no power is worth a drop of blood spilled for it," he said in a statement.
Yet he issued a veiled threat to protesters.
Opposition forces should "disassociate themselves from the radical forces that provoke bloodshed and clashes with law enforcement," he said.
Otherwise, admit to supporting them and be treated accordingly, Yanukovych demanded.
Opposition leaders pointed the finger back, painting their supporters as the victims, not the aggressors.
Neither side seems to have a monopoly on the use of violence, and in the mayhem, it is sometimes hard to tell who is carrying it out.
The journalist who died Wednesday was shot the night before, after a group of masked people stopped a taxi he was riding in, according to a statement by his newspaper Ukrainian Vesti.
They wore camouflage clothes and were throwing Molotov cocktails. They beat other passengers in the car, the paper reported.
Hopes dashed hard
Tuesday's violence followed what seemed like a rare breakthrough.
The government had said it'd drop charges against those arrested in the political unrest.
After holding Kiev's City Hall for three month, protesters pulled back Sunday and unblocked streets in the city center.
But hope died Tuesday, when the speaker of parliament refused to allow amendments that would limit the president's powers.
Opposition anger reignited and poured into the streets.
The government's prosecutor general accused the opposition of breaking "the truce," thus setting the stage for the security crackdown that ensued.
Riot police plowed into the crowd with water cannons, stun grenades and night sticks. Some demonstrators fought back, swinging what looked like baseball bats.
Protesters set fire to the headquarters of the ruling Party of Regions. But the opposition's headquarters, the Trade Unions House, was also smoldering at daybreak Wednesday.
Authorities accused protesters of firing guns at security forces. An armored personnel carrier charged barricades but was quickly inundated set alight.
Kiev was the center of the action, as in the past.
But police said the unrest has spread to western Ukraine, with protesters attacking police and local government offices in a number of regions.
Political fuel, spark
Flaming barricades have been a constant for three months all around Kiev's Maidan, or Independence Square.
But Tuesday's bloodshed marked a decided escalation.
The strife started over a trade pact.
Last year, when President Viktor Yanukovych decided to back away from a trade pact with the European Union. He has instead opted for closer relations to Moscow.
Protesters objected and hit the streets, their anger fueled by underlying sentiments in favor of the West and against Russia.
Police opposed the demonstrators, and their ranks grew in size and fury. Their initial call for Yanukovych to reverse his decision avalanched into an attack on the President's power base.
Yanukovych and his allies responded with some concessions, offering places in government to opposition leaders.
But on-again, off-again talks have gone nowhere.
Both sides have demanded that the other back down first, and neither is budging.
Yanukovych and opposition leader and famed boxer Vitali Klitschko played another round of the you-first-game in an overnight face-to-face meeting.
Speaking to reporters afterward, Klitschko said there effectively was "no discussion."
The President demanded the protesters back off first. Klitschko threw the demand back at him. "I told Yanukovych this," he said. "How can we negotiate when there is blood being spilled?"
Washington vs. Moscow
Since political tensions began, Washington and Moscow have weighed in on opposite ends and kept doing so Tuesday.
U.S. Vice President Joe Biden called Yanukovych to press him to stop the violence, placing the responsibility to de-escalate mainly with government.
Secretary of State John Kerry later backed up the Vice President's words. He called for the Ukrainian government to halt violence immediately, and reopen dialogue with the opposition.
Russia accused Washington of meddling in Ukrainian affairs.
Washington is trying to tell "the authorities of a sovereign state what they should do next and how they should do it," an article in Russia's state-run RIA Novosti's read.
It noted Moscow's persistent support for Yanukovych.
U.N. Sec.-Gen. Ban Ki-moon condemned the clashes in a statement late Tuesday and called for them to stop.
"He reiterates to all sides that the use of violence is unacceptable," it read.
Ban called preventing bloodshed a "paramount priority."
But in Kiev, the call may be falling on the deaf ears of embittered rivals.